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  • Title: Two-dimensional agarose gel electrophoresis without gel manipulation.
    Author: Serwer P.
    Journal: Anal Biochem; 1985 Jan; 144(1):172-8. PubMed ID: 3985312.
    Abstract:
    The apparatus and procedure to perform two-dimensional agarose gel electrophoresis without manipulating the gel used for the first electrophoresis (first-dimension gel) have been developed. The procedure is less complex, less damaging to first-dimension gels, and more precise than procedures that require manipulation of the first-dimension gel. When combined with gel-embedding techniques, the procedure presented can be used to perform the second electrophoresis in a gel different from the first-dimension gel. A first-dimension gel too dilute to be manipulated and a more concentrated gel for the second electrophoresis have been used to separate DNA open circles from a mixture of variable-length linear DNAs.
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