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  • Title: Concentration and purification of influenza virus from allantoic fluid.
    Author: Arora DJ, Tremblay P, Bourgault R, Boileau S.
    Journal: Anal Biochem; 1985 Jan; 144(1):189-92. PubMed ID: 3985314.
    Abstract:
    A simple procedure which enables the concentration and purification of influenza virus, using an angular rotor, is described. Virus is concentrated over a sucrose step gradient. The same gradients are reused and volumes up to 4 liters are concentrated in 1 day. The concentrated virus is further purified by a simplified density-gradient technique. No host cell protein is detectable in the final product. The technique offers a broad application potential for concentrating and purifying other viruses.
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