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  • Title: Quantitative localization of polystyrene microspheres following microinjection in the avian metencephalic neural crest pathway.
    Author: Davis JU, Bringas P, Slavkin HC.
    Journal: J Craniofac Genet Dev Biol; 1985; 5(1):11-9. PubMed ID: 3988888.
    Abstract:
    Polystyrene microspheres were microinjected into crest populations at two preotic sites in Hamburger-Hamilton stage 10 and 11 chick embryos to investigate factors modulating cephalic neural crest fate. Analyses of microsphere localization and comparisons with cephalic crest fate maps indicated the following: microspheres injected at stage 10 localized with derivatives reflecting the fate of the crest population at the injection site; microspheres injected at stage 11 exhibited minimal displacement; and localization in ectoderm was similar in embryos injected at either stage. These results suggest that microinjected microspheres can be used to investigate normal and abnormal craniofacial morphogenesis.
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