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  • Title: [DNA flow cytometric evaluation of spermatogenesis. Part 2: Studies on male infertility].
    Author: Kawai S.
    Journal: Hinyokika Kiyo; 1985 Jan; 31(1):87-95. PubMed ID: 3993498.
    Abstract:
    Flow cytometric DNA analysis was performed on the testicular biopsy tissue obtained from 17 oligospermic men, 25 azoospermic men and 5 normal men. DNA histograms were made after viewing a small piece of biopsy tissue for a short time. The DNA histograms were classified by eye into four types (Type A: Aspermatogenesis without haploid cell, Type B: Maturation arrest at primary spermatocyte, Type C: Hypospermatogenesis, Type D: Normal spermatogenesis). Analysis of the DNA histograms accurately revealed the proportion of haploid, diploid and tetraploid cells. The DNA distributions for 5 normal men were 58.9 +/- 3.6% haploid cells, 24.3 +/- 3.8% diploid cells and 16.8 +/- 0.8% tetraploid cells. Significant correlation was found between the proportion of haploid cells (%haploid) and the testicular volume. The results of the investigation of the correlation between the DNA distributions and histological evaluations show that testicular degeneration increase proportionally to the decrease in haploid cells. Therefore, the %haploid appears to be an effective index for the quantitative evaluation of spermatogenesis.
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