These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Use of digitonin fractionation to determine mitochondrial transmembrane ion distribution in cells during anoxia.
    Author: Andersson BS, Jones DP.
    Journal: Anal Biochem; 1985 Apr; 146(1):164-72. PubMed ID: 3993928.
    Abstract:
    A method to determine intracellular distribution of ions and metabolites under conditions of low oxygen concentration was developed. The technique involves rapid digitonin fractionation and addition of cyanide to inhibit reoxygenation. Applicability of the procedure was examined by studying the distribution of the lipophilic ion triphenylmethylphosphonium, the weak acid 5,5-dimethyloxazolidine-2,4-dione, and adenine nucleotides.
    [Abstract] [Full Text] [Related] [New Search]