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  • Title: Cerulenin inhibition of lipid synthesis and its reversal by exogenous fatty acids in Mycobacterium smegmatis ATCC 607.
    Author: Mahajan S, Khuller GK.
    Journal: Can J Biochem Cell Biol; 1985 Feb; 63(2):85-90. PubMed ID: 3995406.
    Abstract:
    Cerulenin inhibited the lipid synthesis of Mycobacterium smegmatis ATCC 607 over the range of 0.5-1.8 microgram/mL with complete inhibition at 1.8 microgram/mL, as monitored by [14C]glycerol incorporation into lipids. Exogenous fatty acids failed to restore the lipid synthesis at 1.8 microgram/mL; however, the addition of palmitic acid to the growth medium partially restored the lipid synthesis when cerulenin concentration was decreased to 1.6 microgram/mL. Fatty acid analysis of cerulenin plus palmitic acid supplemented cultures revealed that exogenously supplied fatty acid was incorporated into cellular phospholipids. Further investigations with 1.6 microgram/mL of cerulenin and [14C]acetate and [32P]orthophosphoric acid showed that cerulenin inhibited the synthesis of saturated plus unsaturated fatty acids and phospholipids. Pulse-chase studies with [14C]acetate revealed decreased synthesis and degradation of each of the phospholipid components.
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