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  • Title: A monoclonal-antibody-based immunoradiometric assay for apoprotein B in low-density lipoprotein.
    Author: Slater HR, Tindle RW, Packard CJ, Shepherd J.
    Journal: Clin Chem; 1985 Jun; 31(6):841-5. PubMed ID: 3995761.
    Abstract:
    Five different mouse monoclonal antibodies were raised against apoprotein B, the major protein in human low-density lipoproteins (LDL). Competitive binding studies suggested that these antibodies recognized four different epitopes on the lipoprotein particle. All antibodies bound to very-low-density and intermediate-density lipoproteins, which are the metabolic precursors of LDL and also contain apoprotein B. One antibody (P1C1) displayed a relative specificity for apoprotein B in LDL as compared with that in very-low-density lipoprotein and was used as a radiolabeled first antibody in an immunoradiometric assay for this lipoprotein. Measurements, either on isolated LDL or directly on plasma samples, demonstrated that this assay provides a rapid and precise method of quantification.
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