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  • Title: Preservation of integrity of the inner mitochondrial membrane after freeze-thawing and freeze-drying.
    Author: Tsvetkov T, Tsonev L, Meranzov N, Minkov I.
    Journal: Cryobiology; 1985 Jun; 22(3):301-6. PubMed ID: 3996019.
    Abstract:
    The results of this paper illustrate that trehalose partially preserves inner mitochondrial membrane integrity after freeze-thawing and freeze-drying with subsequent rehydration in water. The 2,4-dinitrophenol stimulation of ATPase activity was used as a criterion for membrane integrity. The results show that ATPase activity of lyophilized-rehydrated mitochondria was stimulated up to two to three times.
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