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Title: The structural basis for C-banding. A scanning electron microscopy study. Author: Jack EM, Harrison CJ, Allen TD, Harris R. Journal: Chromosoma; 1985; 91(5):363-8. PubMed ID: 3996103. Abstract: The same C-banded human polymorphic chromosomes were observed in the light microscope (LM) and then in the scanning electron microscope (SEM) to investigate the structural changes produced by the C-banding technique. C-banded regions, which stained positively in LM, were highly condensed with tightly packed chromatin fibres, resembling non-banded chromosomes. In striking contrast, adjacent non-C-banded regions were represented by loosely arranged fibres, resembling G-banded chromosomes. The significance of these observations in relation to current theories on the effects of C-banding on chromosome structure is discussed.[Abstract] [Full Text] [Related] [New Search]