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  • Title: N-Butyrate incubation of immature chicken erythrocytes preferentially enhances the solubility of beta A chromatin.
    Author: Ferenz CR, Nelson DA.
    Journal: Nucleic Acids Res; 1985 Mar 25; 13(6):1977-95. PubMed ID: 4000950.
    Abstract:
    The solubility of adult beta-globin chromatin (beta A chromatin) from immature chicken red blood cells can be controlled by the presence or absence of n-butyrate in a cell incubation medium. In the absence of n-butyrate, only a small percentage (approximately 4%) of the total beta A chromatin is in a soluble chromatin fraction following micrococcal nuclease digestion and centrifugation. This percentage increases to approximately 40-45% of the beta A chromatin if cells are incubated 1 hour in the presence of 10 mM sodium n-butyrate. The highest yield and enrichment of solubilized beta A chromatin is attained when 1-4% of the DNA is rendered acid soluble, and in buffers containing 1.5 - 5 mM MgCl2. The soluble beta A nucleohistone is nucleosome oligomer size (contains DNA 250-600 bases in length) and can be separated from soluble, transcriptionally inert mononucleosomes by agarose A-5m exclusion chromatography. The enhanced solubility appears to be specific for transcriptionally active chromatin. Whereas 40-45% of the beta A chromatin is recovered in the supernatant fraction from n-butyrate incubated immature erythrocytes, nucleohistone containing ovalbumin DNA sequences remains insoluble.
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