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  • Title: Evidence for the Pasteurella haemolytica cytotoxin as a product of actively growing bacteria.
    Author: Shewen PE, Wilkie BN.
    Journal: Am J Vet Res; 1985 May; 46(5):1212-4. PubMed ID: 4003897.
    Abstract:
    The release of soluble cytotoxin from Pasteurella haemolytica type 1 cultured at 37 C in RPMI 1640 medium containing 7% bovine fetal serum was evaluated, using bovine alveolar macrophages in a microplate 51Cr-release assay. Heat-labile leukotoxic activity was detected in culture supernatant during the lag and logarithmic-growth phases, but not in stationary-phase culture; toxic activity could not be demonstrated in sonicates of logarithmic (1 hour) or stationary (18 hour) phase organisms. This pattern of toxin release, typical of a bacterial exotoxin or metabolic enzyme, is unique among bacterial leukotoxins, which are typically cell-associated and released after autolysis or sonication from outgrown cultures.
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