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  • Title: Identification of yeast 60 S ribosomal proteins crosslinked to rRNA by 2-iminothiolane.
    Author: el-Baradi TT, Raué HA, Linnekamp M, Planta RJ.
    Journal: FEBS Lett; 1985 Jul 01; 186(1):26-30. PubMed ID: 4007157.
    Abstract:
    Saccharomyces carlsbergensis 60 S ribosomal subunits were treated with the hetero-bifunctional crosslinking agent 2-iminothiolane and then subjected to mild UV irradiation to introduce protein-rRNA crosslinks. The major crosslinked products were identified as proteins L2, L3, L5, L19 and L23 of which L5 was found to be crosslinked at a 3-5-fold higher efficiency than the other four. Several additional proteins were cross-linked to a detectable but much lower extent.
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