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  • Title: Characterization of mitochondrial aspartate aminotransferase from the liver of pyridoxine-deficient rats.
    Author: Nagata K, Okada M.
    Journal: J Biochem; 1985 Feb; 97(2):501-7. PubMed ID: 4008465.
    Abstract:
    The properties of crude and purified mitochondrial aspartate aminotransferase preparations from pyridoxine-deficient and control rat livers were compared. The preparations from the two sources showed very similar behaviors on heat treatment, electrophoresis and chromatofocusing, and had similar molecular weights, but their visible absorption spectra and circular dichroism properties were different. These results suggest that mitochondrial aspartate aminotransferase from pyridoxine-deficient and control rat livers have very similar properties, but differ somewhat in conformation in the region of the pyridoxal phosphate binding site.
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