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  • Title: Synthesis of (+) and (-) RNA molecules of potato spindle tuber viroid (PSTV) in isolated nuclei and its impairment by transcription inhibitors.
    Author: Spiesmacher E, Mühlbach HP, Tabler M, Sänger HL.
    Journal: Biosci Rep; 1985 Mar; 5(3):251-65. PubMed ID: 4016225.
    Abstract:
    Transcription studies with highly purified potato cell nuclei in combination with a 'transcription-hybridization analysis' unequivocally demonstrate that the nucleus is the subcellular site where the entire process of PSTV replication takes place. Inhibition experiments with actinomycin D and alpha-amanitin furthermore suggest that the nuclear DNA-dependent RNA polymerases I and II are involved in the synthesis of PSTV (+) and (-) RNA, respectively.
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