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  • Title: Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test.
    Author: Terstappen LW, de Grooth BG, Nolten GM, ten Napel CH, van Berkel W, Greve J.
    Journal: Cytometry; 1985 Jul; 6(4):316-20. PubMed ID: 4017797.
    Abstract:
    A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG. The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.
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