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  • Title: Induction of spheroplasts in Capnocytophaga ochracea.
    Author: Odgers J, Dyer JK, Martin EL, Bolton RW, Ang BJ.
    Journal: J Gen Microbiol; 1985 May; 131(5):993-9. PubMed ID: 4020345.
    Abstract:
    A gentle technique for preparing spheroplasts of Capnocytophaga ochracea strain 25 is described. Cells in the exponential phase were washed with 1.0 M-NaCl, agitated in 1.0 M-NaCl for 2 h at 30 degrees C and exposed to lysozyme in a Tris/salts buffer, pH 7.0. This procedure resulted in 98% spheroplast formation with complete removal of the peptidoglycan layer as detected by both phase-contrast and electron microscopy in combination with chemical analysis.
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