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  • Title: [Characterization of the "4 S-trypsin" form of the cytosoluble estradiol receptor of calf uterus in a nondenaturing system].
    Author: Secco-Millet C, Soulignac O, Rocher P, Baulieu EE, Richard-Foy H.
    Journal: C R Acad Hebd Seances Acad Sci D; 1977 Jan 10; 284(2):137-40. PubMed ID: 402239.
    Abstract:
    The characterisation of the "4 S-trypsin" form of the estradiol-receptor from calf uterus cytosol was carried out by polyacrylamide gel electrophoresis under non-denaturing conditions. In a multiphasic buffer system (upper buffer Tris-glycine pH25degrees C = 8,6, lower buffer Tris HCl pH25degrees C = 7,4), three radioactive estradiol peaks were observed. The first was free estradiol, the second estradiol initially complexed with a macromolecule and dissociated during electrophoresis, and the third the hormone-receptor complex. The sedimentation coefficient (4 S) of this complex was the same before and after electrophoresis. Its mean geometric radius is R = 2.53 + 0.08 nm and its molecular weight was estimated to be 55,000.
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