These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Ether-phospholipid composition in neutrophils and platelets.
    Author: Tencé M, Jouvin-Marche E, Bessou G, Record M, Benveniste J.
    Journal: Thromb Res; 1985 May 01; 38(3):207-14. PubMed ID: 4024039.
    Abstract:
    It is well documented that ether-lipids, especially the 1-0-alkyl-2-0-acyl-sn-glycero-3-phosphocholine, can serve as precursors in paf-acether (platelet-activating factor) biosynthesis. This study was undertaken to determine the amount of these compounds in two cell types which are good producers of paf-acether: human neutrophils and rabbit platelets. The method of phospholipid analysis was based on selective destruction of diacyl molecules by lipase from guinea pig pancreas and of plasmalogens by acidolysis; phospholipids were then separated by bidimensional thin-layer chromatography. In platelets as in neutrophils, the major phospholipids were choline (37%) and ethanolamine (30 and 32%, respectively) phosphoglycerides and sphingomyelin (18 and 16%, respectively). In rabbit platelets the 1-alkyl molecules represented 15% of the choline class (0.7 nmol/10(7) cells). Neutrophils differed strikingly from platelets by the high level of these molecules which constituted 50% of the choline class (16.3 nmol/10(7) cells). It therefore appears that in the two cell types, the amount of 1-0-alkyl-2-0-acyl-sn-glycero-3-phosphocholine largely exceeded that would be necessary for paf-acether biosynthesis.
    [Abstract] [Full Text] [Related] [New Search]