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  • Title: [Ultrastructure of hematopoietic and stromal cells of the subendosteal region of the bone marrow].
    Author: Butenko ZA, Zak KP, Khomenko BM, Afanas'eva VV.
    Journal: Tsitologiia; 1985 Jun; 27(6):628-32. PubMed ID: 4024255.
    Abstract:
    Using electron microscopic cytochemistry and immunoelectron microscopy, the ultrastructure of bone marrow (BM) cells of the subendosteal region with a high colony-forming (CFUs) ability was studied. In comparison with the central part of BM, the subendosteal region of CBA and BALB/c mice contains a higher number of lymphocyte-like mononuclears, bearing an antigen, common with the brain surface one but negative for peroxidase and acid and alkaline phosphatase. The ultrastructure of these cells is similar to that of presumptive hematopoietic stem cells. In the subendosteal region mononuclears are concentrated with the lower nucleo-cytoplasmic ratio, a feston-like line of the nucleus and more numerous organoids. These cells are characteristic of BM myeloid islands composed of granulocytes being on various stages of differentiation, and of reticular cells positive for alkaline phosphatase.
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