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  • Title: Isolation and partial characterization of a novel basement membrane collagen.
    Author: Dixit R, Harrison MW, Dixit SN.
    Journal: Biochem Biophys Res Commun; 1985 Jul 16; 130(1):1-8. PubMed ID: 4026821.
    Abstract:
    A guanidine-HCl extraction of lens capsule basement membrane dissolves collagenous material. This material was fractionated on an Agarose A-5M column. Fractions 1, 2 and 3 were further purified and partially characterized immunochemically and by amino acid analysis. Fraction 3 has a molecular weight of 55,000 when compared with collagen type I standard. The CNBr peptide pattern and composition of fraction 3 are different from those of alpha 1 (IV) 95K and alpha 2 (IV) 95K chains. The results described suggest the presence of a new chain in lens capsule basement membrane.
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