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  • Title: Selenium-dependent and non-selenium-dependent glutathione peroxidase in human tissues of New Zealand residents.
    Author: Thomson CD.
    Journal: Biochem Int; 1985 Apr; 10(4):673-9. PubMed ID: 4026872.
    Abstract:
    Glutathione peroxidase was assayed in human tissues of New Zealand residents by the coupled assay method. Total glutathione peroxidase was assayed using cumene hydroperoxide. The non-selenium-dependent activity was not detected with t-butyl hydroperoxide and thus was determined from the difference between total activity and the selenium-dependent activity using hydrogen peroxide or t-butyl hydroperoxide. Only selenium-dependent activity was found in whole blood, erythrocytes, platelets and biopsy skeletal muscle. A small non-selenium dependent activity was measured in plasma and a larger activity in biopsy liver supernatant and homogenate. Glutathione-S-transferase was detected in all tissues.
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