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  • Title: Hemocyanins in spiders, XX. Sulfhydryl groups and disulfide bridges in subunit d of Eurypelma californicum hemocyanin.
    Author: Eyerle F, Schartau W.
    Journal: Biol Chem Hoppe Seyler; 1985 Apr; 366(4):403-9. PubMed ID: 4026994.
    Abstract:
    Subunit d of Eurypelma californicum hemocyanin contains after reduction 7 cysteine residues. Using 3,3'-dithiobis(6-nitrobenzoic acid) 3 mol cysteine/mol subunit were determined. The cysteine- and cystine-containing peptides of subunit d were obtained by cyanogen bromide cleavage and subsequent treatment with trypsin. The free cysteines were established at positions 102, 261, and 454 respectively. Cys205-Cys210 and Cys529-Cys579 are connected by disulfide bridges.
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