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  • Title: [Characteristics of a cell line obtained from a human hypernephroma].
    Author: Shevliagin VIa, Shaposhnikova GM, Snegireva AE, Chizhevskaia VI, Miller GG.
    Journal: Nauchnye Doki Vyss Shkoly Biol Nauki; 1985; (6):29-33. PubMed ID: 4027291.
    Abstract:
    A stable tumorigenic cell line has been obtained from the human hypernephrome. The culture grew as monolayers of large polygonal cells with foamy cytoplasm and 1-2 nucleoli. The cells formed monolayer after the third day after planting into flask and then formed multilayered growth. The electron microscopic studies of hypernephrome sections revealed the dark and light cells. The hypernephrome cells have characteristics of tumor cells. On the 200th passage the cells are aneuploid with a model number of 62 chromosomes; an acrocentric chromosome in D group is noted as a marker one. The hypernephrome cells are free of Mycoplasma contamination, able to support the several virus replication.
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