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  • Title: Thin layer chromatographic determination of sterigmatocystin in cheese.
    Author: Francis OJ, Ware GM, Carman AS, Kuan SS.
    Journal: J Assoc Off Anal Chem; 1985; 68(4):643-5. PubMed ID: 4030632.
    Abstract:
    A one-dimensional thin layer chromatographic method has been developed for determining sterigmatocystin in cheese. Cheese is extracted with acetonitrile-4% KCl (85 + 15). A simplified liquid-liquid partition cleanup is used, and the sample extract is passed through a cupric carbonate column for final purification. Sterigmatocystin is visualized by spraying the plate with aluminum chloride. The fluorescence of the spot is enhanced 10-fold by additional plate spraying with a silicone-ether mixture, enabling sterigmatocystin detection and quantitation at 2 and 5 micrograms/kg, respectively. Average recoveries were 88.3 and 86.4% at the 10 and 25 micrograms/kg levels, respectively.
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