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  • Title: Crystallization of calcium-calmodulin-trifluoperazine complex and an attempt at crystallizing calcium-free calmodulin.
    Author: Kawasaki H, Itoh S, Kasai H, Mitsui Y, Iitaka Y, Nakanishi M, Okuyama T.
    Journal: J Biochem; 1985 Jun; 97(6):1815-8. PubMed ID: 4030751.
    Abstract:
    Crystals suitable for X-ray structure analysis were obtained for CaM complexed with both calcium ions and a phenothiazine drug, trifluoperazine (TFP). The TFP/CaM binding ratio in crystals was experimentally determined to be nearly 1. An attempt at crystallizing calcium-free calmodulin (CaM) resulted in rigid but non-birefringent solids which exhibited no X-ray reflections.
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