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  • Title: [Use of monoclonal antibodies in the diagnosis of fibrinolysis].
    Author: Wojta J, Korninger C, Kirchheimer J, Hattey E, Turcu L, Binder BR.
    Journal: Wien Klin Wochenschr; 1985 Mar 01; 97(5):244-8. PubMed ID: 4039506.
    Abstract:
    Monoclonal antibodies to tissue type plasminogen activator (t-PA), urokinase (u-PA) and alpha-2-antiplasmin were obtained by immunizing Balb C mice with the respective purified antigens and fusion of spleen cells with mouse myeloma cells (NSO). The selected monoclonal antibodies reacted exclusively with the respective antigens used for immunization. 3 out of 7 monoclonal antibodies directed against t-PA inhibited plasminogen activation by t-PA and 6 of 7 interfered with the fibrin potentiating effect. Only one out of 5 monoclonal antibodies directed against u-PA inhibited plasminogen activation by u-PA. None of the monoclonal antibodies directed against t-PA or u-PA inhibited the cleavage of low molecular weight paranitroanilide substrates by the respective plasminogen activators. Likewise, the 3 monoclonal antibodies to alpha-2-antiplasmin did not functionally inhibit alpha-2-antiplasmin. The selected monoclonal antibodies were used to develop sensitive test systems for the respective antigens. The lower detection limit for determination of t-PA was found to be 0.5 ng/ml, for determination of u-PA 0.05 ng/ml, and for determination of alpha-2-antiplasmin 5 ng/ml.
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