These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Partial purification and characterization of a vascular permeability factor secreted by a human colon adenocarcinoma cell line.
    Author: Lobb RR, Key ME, Alderman EM, Fett JW.
    Journal: Int J Cancer; 1985 Oct 15; 36(4):473-8. PubMed ID: 4044056.
    Abstract:
    The established human colon adenocarcinoma cell line, HT-29, secretes a vascular permeability factor (VPF) in vitro. The factor has been purified from serum-free conditioned medium by acidification, cation-exchange, and reverse-phase and anion-exchange high-performance liquid chromatography. The VPF is a non-glycosylated acidic protein of apparent molecular weight 45,000, and is at least 4 orders of magnitude more potent than histamine as an inducer of vascular permeability. Its biological activity is unaffected by soybean trypsin inhibitor or by inhibitors of histamine, kinins, prostaglandins, or acid proteases. The VPF induces vascular permeability within minutes, which suggests a direct effect on the endothelial cell.
    [Abstract] [Full Text] [Related] [New Search]