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  • Title: Formation of a fluorescent glucocorticoid receptor-steroid complex in HTC cell cytosol.
    Author: Pons M, Robinson TE, Mercier L, Thompson EB, Simons SS.
    Journal: J Steroid Biochem; 1985 Sep; 23(3):267-73. PubMed ID: 4046600.
    Abstract:
    An intensely fluorescent rhodamine derivative of dexamethasone (i.e. Dex-C2-Rho) was synthesized. Dex-C2-Rho possessed high affinity for HTC cell glucocorticoid receptors in cell-free systems. Whole cell activity and receptor affinity of Dex-C2-Rho were both much lower, apparently due to problems with cell permeability and/or metabolism. A specific, fluorescent receptor-steroid complex at concentrations as low as 1 X 10(-9) M could readily be observed with crude HTC cell receptors after removal of the free Dex-C2-Rho. This appears to be the first report of a fluorescent glucocorticoid receptor-steroid complex.
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