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  • Title: Determination of the polyamine content of rat heart mitochondria by the use of heparin-sepharose.
    Author: Tadolini B, Cabrini L, Piccinini G, Davalli PP, Sechi AM.
    Journal: Appl Biochem Biotechnol; 1985 Jun; 11(3):173-6. PubMed ID: 4051476.
    Abstract:
    Heparin-sepharose has been utilized to remove polyamines adsorbed to the cytoplasmic surface of rat heart mitochondria. The results obtained can be summarized as follows: Heparin-sepharose removes 90% of the spermine, 98% of the spermidine, and 98% of the putrescine adsorbed. Polyamine contents of chromatographed mitochondria amount to 2.66 and 0.36 nmol spermine and spermidine, respectively, per mg of mitochondrial protein.
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