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  • Title: Heat-shock treatment lethal for mammalian cells deprived of glucose and glutamine: protection by alpha-keto acids.
    Author: Gomes MI, Kim WJ, Lively MK, Amos H.
    Journal: Biochem Biophys Res Commun; 1985 Sep 16; 131(2):1013-9. PubMed ID: 4052064.
    Abstract:
    Nil and Nilpy hamster cells exposed to temperatures of 44 degrees C to induce the heat-shock proteins survive such exposure for 2 h or more when incubated in Eagle's Minimum Essential Medium with 10% undialyzed fetal calf serum. If D-glucose and L-glutamine are withdrawn from the medium during heat treatment, nearly all the cells are killed by as little as 20 min at 44 degrees C. Several alpha-keto acids, pyruvate, alpha-ketobutyrate, oxaloacetate, and alpha-ketoglutarate, protect cells from the lethal action of the heat treatment in the absence of D-glucose and L-glutamine. L-Glucose and D-glutamine are without effect. Efforts to reverse lethal effects have not been successful.
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