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  • Title: [Characteristics of palindromic sequences in DNA of the sea urchin Strongylocentrotus intermedius].
    Author: Brykov VA, Kukhlevskiĭ AD.
    Journal: Biokhimiia; 1985 Sep; 50(9):1553-9. PubMed ID: 4052511.
    Abstract:
    Some properties of the palindromic sequences in the sea urchin Strongylocentrotus intermedius nuclear DNA have been studied. It was shown that the amount of "foldback HAP bound DNA" and the S1 nuclease resistant DNA depends on renaturation temperature and Na+ concentration in solution. The authentic fraction of inverted repeats comprises 10-15% of the total DNA. The complexity of the palindromic fraction is approximately 8,2 X 10(7) nucleotide pairs and the average number of inverted repeats approximates 5 X 10(5) per haploid genome. The renaturation kinetics of inverted repeats with excess of total homologous DNA indicates that these sequences are enriched with unique DNA. The possible function of palindromic sequences is discussed.
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