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  • Title: Evaluation of equilibrium gel filtration chromatography for the study of protein binding of aluminum in normal and uremic sera.
    Author: Bertholf RL, Wills MR, Savory J.
    Journal: Clin Physiol Biochem; 1985; 3(5):271-6. PubMed ID: 4053493.
    Abstract:
    Normal and uremic serum samples were chromatographed on a gel filtration column and fractions were analyzed for protein and aluminum content. Whereas much of the aluminum in the serum samples eluted with protein, a significant fraction appeared to be associated with low molecular weight species. Analysis of column fractions for aluminum binding by a competitive binding assay indicated no aluminum-binding serum constituents in these later eluting fractions. Uremic serum appeared, however, to contain aluminum-binding constituents of a lower molecular weight than in normal serum.
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