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  • Title: Isolation and characterization of a mucin-glycoprotein from rat submandibular glands.
    Author: Tabak LA, Mirels L, Monte LD, Ridall AL, Levine MJ, Loomis RE, Lindauer F, Reddy MS, Baum BJ.
    Journal: Arch Biochem Biophys; 1985 Nov 01; 242(2):383-92. PubMed ID: 4062287.
    Abstract:
    A blood group A+ mucin-glycoprotein was purified from aqueous extracts of rat submandibular glands by sequential chromatography on columns of Sepharose CL-6B and Sephacryl S-300 in urea-containing buffers. Final purification was facilitated by reductive methylation which appeared to release contaminating (hydrophobic) peptides. Homogeneity of the purified mucin was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at varying concentrations of acrylamide, lectin affinity chromatography, and Western blot analysis. In contrast to previously described preparations, the purified mucin contained only trace amounts of N-acetylglucosamine and aromatic amino acids. In addition, only low levels of basic amino acids were present.
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