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  • Title: Repigmentation of human retinal pigment epithelial cells in vitro.
    Author: Boulton M, Marshall J.
    Journal: Exp Eye Res; 1985 Aug; 41(2):209-18. PubMed ID: 4065245.
    Abstract:
    Cultured human retinal pigment epithelial (RPE) cells readily ingested both melanin and lipofuscin isolated from human RPE cells. Up to 7 days post-challenge ingested granules demonstrated no evidence of lysis or aggregation within secondary lysosomes. When cultures containing ingested melanin and lipofuscin were subcultured the cells gradually depigmented due to a redistribution of pigment granules amongst daughter cells. Quantitative analysis demonstrated that the accumulation of both types of granule was linear over a 24 hr challenge period. This study reports a technique of 'artificially' repigmenting cultured human RPE cells and thus offers the potential for in vitro investigations of the role of these inclusions in various dynamic aspects of cellular metabolism.
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