These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Interaction between human polymorphonuclear leucocytes and Chlamydia trachomatis elementary bodies: electron microscopy and chemiluminescent response.
    Author: Zvillich M, Sarov I.
    Journal: J Gen Microbiol; 1985 Oct; 131(10):2627-35. PubMed ID: 4067575.
    Abstract:
    Incubation of human polymorphonuclear leucocytes (HPMN) with highly purified Chlamydia trachomatis serotype L2/434/Bu elementary bodies (EB), in the presence and absence of specific antibody, resulted in a 10(3)-fold reduction of viable count after 24 h incubation. Electron microscopy observations indicated activation of the HPMN by the EB. Attachment of the EB to the HPMN cell membrane, formation of a cytoplasmic cup and EB-containing vacuoles were observed. In addition, two types of phagocytic vacuoles were observed after 30 min incubation; in one type, a single EB was tightly surrounded by the vacuolar membrane, while the other type was enlarged and held one or more intact EB or degenerated EB or both. A fuzzy coat was observed on EB located in the HPMN vacuoles only in the presence of specific antibody. Empty vacuoles containing degenerated EB were observed in the HPMN after 24 h incubation. HPMN exposed to EB of C. trachomatis produced a marked chemiluminescent response with a peak 14 times greater than the peak value of the control. A second stimulation with phorbol 12-myristate 13-acetate and zymosan was achieved. The chemiluminescent peak value in the presence of heat-treated EB (56 degrees C, 20 min) was 50% of that obtained in the presence of untreated EB. The significance of the chemiluminescent response in the killing mechanism of C. trachomatis EB by HPMN is discussed.
    [Abstract] [Full Text] [Related] [New Search]