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  • Title: Oxidant effect of acetylphenylhydrazine: a comparative study with erythrocytes of several animal species.
    Author: Kurian M, Iyer GY.
    Journal: Can J Biochem; 1977 Jun; 55(6):597-601. PubMed ID: 406973.
    Abstract:
    Red cells from several animal species were treated with acetylphenylhydrazine and a comparative study of the oxidation of hemoglobin (Hb) and glutathione (GSH) made. Wide interspecies differences were observed but the oxidation of GSH paralleled that of Hb. Added glucose protected both Hb and GSH from oxidation; GSH by itself exercises a protective effect on Hb. The characteristic rates of oxidation of GSH in the different species can be observed only in the presence of oxyhemoglobin but not carboxyhemoblobin or methemoglobin. The oxidation of Hb appears to be the primary event, the oxidation of GSH being a consequence thereof.
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