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  • Title: Identification of the bicarbonate secretory cell of the turtle bladder.
    Author: Fritsche C, Schwartz JH.
    Journal: Am J Physiol; 1985 Dec; 249(6 Pt 2):F858-62. PubMed ID: 4073269.
    Abstract:
    The turtle bladder consists primarily of two mucosal cell types, mitochondrial rich (MR) and granular (G) cells. The MR cells secrete H+. In addition, the bladder secretes HCO3-, an energy-requiring Cl(-)-dependent transport system. These studies were designed to identify the cell type responsible for HCO3- secretion. Specific HCO3- and H+ secretory inhibitors were added to alter O2 consumption of MR and G cells. Cells were separated by Ficoll density centrifugation. Ouabain (10(-4) M) was used in all studies to eliminate O2 consumption associated with Na+ transport. O2 consumption of cells treated with 10(-4) M 4-acetamido-4'-isothiocyanostilbene-2,2'-sulfonic acid (SITS), which indirectly inhibits H+ secretion, was compared with cells additionally treated with 5 X 10(-4) M acetazolamide, an inhibitor of both H+ and HCO3- secretion. O2 consumption of MR cells treated with SITS alone was significantly greater than that of MR cells additionally treated with acetazolamide (delta = 0.56 +/- 0.10 microliter O2 X h-1 X mg protein-1, n = 6, P less than 0.003). There was no significant difference in G cells similarly treated. Another means of selectively altering H+ and HCO3- transports and their associated metabolic rates is to manipulate the Cl- concentration of the incubation medium. In a Cl(-)-rich medium, HCO3- and H+ transports are maximized. In low Cl(-)-SO2-4 medium, HCO3- secretion is selectively reduced.(ABSTRACT TRUNCATED AT 250 WORDS)
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