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  • Title: Rapid method for determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography.
    Author: Kubo H, Kobayashi Y, Nishikawa T.
    Journal: Antimicrob Agents Chemother; 1985 Oct; 28(4):521-3. PubMed ID: 4073874.
    Abstract:
    A rapid, simple, and accurate method for the determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography is described. The serum proteins were precipitated with 3.5% perchloric acid containing sodium octanesulfonate. After centrifugation, a sample of the supernatant was directly injected into the chromatograph. The determination of kanamycin and dibekacin was performed by a combination of reverse-phase, ion-pair chromatography, postcolumn derivatization with o-phthalaldehyde, and fluorescence detection. The correlation coefficients with fluorescence polarization immunoassay were 0.996 for kanamycin and 0.957 for dibekacin.
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