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  • Title: [Role of the transmembrane potential in impairing the barrier properties of erythrocyte membranes during cryopreservation].
    Author: Gulevskiĭ AK, Riazantsev VV, Kukushkin AI.
    Journal: Biull Eksp Biol Med; 1985 Dec; 100(12):690-1. PubMed ID: 4074868.
    Abstract:
    Exposure of erythrocytes to nonpermeable cryoprotector solution (PEO-1500, sucrose) with reduced NaCl content resulted in the development of chlorine diffusion potential, calculated by the degree of medium acidulation. This was accompanied by the loss of intracellular K+ and the increase in hemolysis upon freezing and thawing.
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