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  • Title: Alteration in calcium-binding activity in synaptosomal membranes from rat brains in association with physical dependence upon ethanol.
    Author: Virmani M, Majchrowicz E, Swenberg CE, Gangola P, Pant HC.
    Journal: Brain Res; 1985 Dec 16; 359(1-2):371-4. PubMed ID: 4075156.
    Abstract:
    The effects of ethanol treatment on calcium-binding activity in synaptosomal membrane fraction from rat brains were studied. The synaptosomal membrane fraction from the hippocampus, the cortex and the cerebellum from control, single dose (6 g/kg), dependent intoxicated (prodromal) and dependent withdrawing (ethanol withdrawal syndrome) rats were used. Two different methods were used for determining the calcium activity in these membrane preparations: the calcium chelator fluorescence probe, chlortetracycline (CTC), was used to measure Ca2+ binding sites in the membranes, and radioactive calcium (45Ca) was used to measure the calcium binding to the synaptosomal membranes. Both methods provided similar conclusions; the calcium activity was higher during the dependent intoxicated phase of the ethanol withdrawal period. The synaptosomal membranes from the hippocampus showed more drastic changes in calcium-binding activity than the cortex and the cerebellum. These results suggest that ethanol dependence is associated with changes in calcium-binding activity in certain areas of the rat brain.
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