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  • Title: The incorporation of O6-methyldeoxyguanosine monophosphate and O4-methyldeoxythymidine monophosphate into polynucleotide templates leads to errors during subsequent in vitro DNA synthesis.
    Author: Saffhill R, Hall JA.
    Journal: Chem Biol Interact; 1985 Dec 31; 56(2-3):363-70. PubMed ID: 4075456.
    Abstract:
    O4-MedTMP and O6-MedGMP may be incorporated from their 5'-triphosphates into polynucleotides by DNA polymerases. Once incorporated O6-MedGMP leads to errors during further cycles of DNA synthesis with a mutagenic efficiency of 0.3-0.7, the value depending upon the relative dNTP concentrations in the assay. In contrast O4-MedTMP leads to very few errors following its incorporation into DNA. The results demonstrate that O6-MedGMP, once incorporated into DNA, should be destined to give rise to DNA base changes during the second round of DNA synthesis.
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