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  • Title: Normal-phase high-performance liquid chromatographic separation of non-derivatized ganglioside mixtures.
    Author: Gazzotti G, Sonnino S, Ghidoni R.
    Journal: J Chromatogr; 1985 Dec 04; 348(2):371-8. PubMed ID: 4077945.
    Abstract:
    A new analytical and semi-preparative high-performance liquid chromatographic method for the separation of a brain ganglioside mixture into individual components is described. Gangliosides were applied to a LiChrosorb-NH2 column and eluted with the solvent system acetonitrile-phosphate buffer at different volume ratios and ionic strengths. The elution profile was monitored by flow-through detection of UV absorbance at 215 nm. The separation of mono- to polysialogangliosides was performed in one step in a total elution time lower than 90 min and with high reproducibility.
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