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  • Title: Mutants of Bacillus megaterium resistant to uncouplers of oxidative phosphorylation.
    Author: Decker SJ, Lang DR.
    Journal: J Biol Chem; 1977 Sep 10; 252(17):5936-8. PubMed ID: 408344.
    Abstract:
    Mutants of Bacillus megaterium displaying malate-stimulated ATP synthesis resistant to uncouplers of oxidative phosphorylation were isolated and partially characterized. ATP synthesis in such mutants was resistant to carbonyl cyanide m-chlorophenyl hydrazone as well as to other uncouplers including 2,4-dinitrophenol, pentachlorophenol, and sodium azide. ATP synthesis in the wild type and in resistant mutants was sensitive to N,N'-dicyclohexylcarbodiimide, tributyltin, valinomycin plus potassium, and potassium cyanide. Active transport of glycine and glutamine which are sensitive to uncouplers in the wild type was also uncoupler-sensitive in the mutants.
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