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  • Title: Qualitative organic analysis. I. Identification of drugs by principal components analysis of standardized thin-layer chromatographic data in four eluent systems.
    Author: Musumarra G, Scarlata G, Cirma G, Romano G, Palazzo S, Clementi S, Giulietti G.
    Journal: J Chromatogr; 1985 Dec 20; 350(1):151-68. PubMed ID: 4093478.
    Abstract:
    Principal component analysis of standardized RF values in four eluent systems [ethyl acetate-methanol-30% ammonia (85:10:15), cyclohexane-toluene-diethylamine (65:25:10), ethyl acetate-chloroform (50:50) and acetone, with the plate dipped in potassium hydroxide solution] provided a two-component model which accounts for 73% of the total variance. The "scores" plot allowed the restriction of the range of inquiry to a few candidates. This result is of great practical significance in analytical toxicology, especially when account is taken of the cost, the time, the analytical instrumentation and the simplicity of the calculations required by the method.
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