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  • Title: Solubility and structure of domains of chicken erythrocyte chromatin containing transcriptionally competent and inactive genes.
    Author: Komaiko W, Felsenfeld G.
    Journal: Biochemistry; 1985 Feb 26; 24(5):1186-93. PubMed ID: 4096899.
    Abstract:
    Chromatin generated by micrococcal nuclease digestion of erythrocyte nuclei can be fractionated into two pools of differing solubility in solvents containing 0.15-0.25 M NaCl. A fixed percentage of the chromatin is soluble under these conditions, independent of the average size of the DNA in the unfractionated chromatin. Chromatin containing particular gene sequences is also distributed between soluble and insoluble fractions in a way that is independent of the average size of the starting material. However, the actual percentage of gene copies present in each fraction is not necessarily the same as for bulk chromatin. The transcriptionally active chicken erythrocyte adult beta-globin gene is more soluble than the bulk, while the ovalbumin gene in the same tissue is less soluble. These differences do not appear to be related to variations in content of RNA, core histones, or two classes of non-histone proteins. Instead, we find that the soluble chromatin pool is somewhat depleted in histones H1 and H5 and contains lower molecular weight DNA than precipitable chromatin. The soluble fraction can be made insoluble by addition of H1. If the precipitable chromatin fraction is redigested to reduce its size and then recombined with the soluble fraction and reprecipitated, the distribution of globin gene is randomized. The results suggest that the partitioning of chromatin into soluble and insoluble pools in 0.15-0.25 M NaCl arises from redistribution of a limiting amount of histones H1 and H5 to the chromatin fractions containing the longest DNA.
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