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  • Title: Radioautography of the optic tectum of the goldfish after intraocular injection of ( 3 H)proline.
    Author: Neale JH, Neale EA, Agranoff BW.
    Journal: Science; 1972 Apr 28; 176(4033):407-10. PubMed ID: 4112669.
    Abstract:
    Radioautography of the optic tectum of the goldfish, performed after injection of [(3)H]proline into the contralateral eye, effectively resolves several distinct layers of retinal synapses. Silver grains are found unilaterally over nerve tracts containing efferent fibers from the tectum, a result that suggests intercellular migration of labeled molecules. The low background and high specific grain density obtained with [(3)H]proline radioautography indicate the usefulness of this technique for the elucidation of neuroanatomical connections in the visual system.
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