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  • Title: Effect of trichloroacetic acid treatment on certain properties of spores of Bacillus cereus T.
    Author: Shibata H, Uchida M, Hayashi H, Tani I.
    Journal: Microbiol Immunol; 1979; 23(5):339-47. PubMed ID: 41162.
    Abstract:
    Spores of Bacillus cereus T treated with trichloroacetic acid (6.1--61.2 mM) were compared with untreated spores, and as the concentration of the chemical increased, the following alterations in spore properties were found: (1) the extent of germination decreased irrespective of the germination medium used; (2) the spores became sensitive to sodium hydroxide (1 N) and hydrochloric acid (0.27 N), but not to lysozyme (200 micrograms/ml); (3) loss of dipicolinate increased on subsequent heating; and (4) the spores became more sensitive to heat. However, trichloroacetic acid-treated spores were still viable and there was no significant change in spore components. The mechanism of action of trichloroacetic acid is discussed.
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