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  • Title: The use of single-radial-haemolysis for rubella antibody studies.
    Author: Clarke M, Boustred J, Seagroatt V, Schild GC.
    Journal: J Hyg (Lond); 1977 Dec; 79(3):355-64. PubMed ID: 411831.
    Abstract:
    The use of a single-radial-haemolysis technique for the detection of antibody to rubella virus is described. The single-radial-haemolysis test was compared with the standard HI methods for the detection of antibody to rubella virus. A close correlation between the two methods was observed in a survey of over two thousand serum samples and the study indicated that single-radial-haemolysis was highly satisfactory as an assay method for IgG antibodies to rubella virus. It was found that the immuno-globulins active in SRH tests sedimented in the 7S range in sucrose rate gradients and were presumably immunoglobulins of the IgG class, but 19S immunoglobulins did not produce haemolysis.
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