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  • Title: Ultrastructural study of poly- -hydroxybutyrate granules from Bacillus cereus.
    Author: Dunlop WF, Robards AW.
    Journal: J Bacteriol; 1973 Jun; 114(3):1271-80. PubMed ID: 4123236.
    Abstract:
    The freeze-etching technique was used to examine the effects of fracturing and etching on the appearance of poly-beta-hydroxybutyrate granules from Bacillus cereus. These granules were examined in extracts isolated by hypochlorite or by sonic treatment, and also in fixed and unfixed intact cells; in the latter case they were compared with granules in thin sections of intact cells. After freeze-fracturing, the diameter of the granules in intact cells was between 240 and 720 nm. The granules consisted of a central core, of diameter between 140 and 370 nm, which occupied less than 50% of the volume of the granule and which was either stretched or removed on fracturing; the core was surrounded by an outer coat which may be bounded by a membrane.
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