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  • Title: Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.
    Author: Gerber H, Horisberger M, Bauer H.
    Journal: Infect Immun; 1973 Mar; 7(3):487-92. PubMed ID: 4123394.
    Abstract:
    A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.
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